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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis/NIR. As attracted Fig - https://urlscan.io/result/3823bc3a-74b6-4d0f-8f09-522e983b4d26/. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate


l9 As shown in Fig. 9, this could be due to the fact that the H4B radical function as an electron sink in a later point in the reaction, so regarding enable NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.